What is the Benedict's test for reducing sugars?
A commonly used test to detect carbohydrates is the Benedict's test for reducing sugars such as glucose and fructose. Benedict's reagent, containing sodium bicarbonate, sodium citrate, and copper sulfate, is added to a solution and heated.
The Benedict's test identifies reducing sugars based on their ability to reduce the cupric ions to cuprous oxide at high pH values (basic solutions). Cuprous oxide is green to reddish orange. A green solution indicates a small amount of reducing sugars, while a reddish-orange solution indicates an abundance of reducing sugars. If the solution contains sucrose, a nonreducing sugar, there is no change in color in the solution, and it remains blue.
Benedict's test for reducing sugars....